![]() Particular gene is all CCLE lines with data for that gene. The reference population used to compute the median and MAD for a The "robust" z-score is resistant to outliers by using median instead of mean and median absolute deviation (MAD) instead of standard deviation. Z-scores: The number of standard deviations that a gene is above or below the population mean is called its z-score. Post-normalization, the expression values range between 4 and 15 log 2 units, with 4 indicating thatĪ gene is minimally or not expressed and 15 indicating the maximum readout. ![]() Such that the expression values were comparable across cell lines, by minimizing technical variationĪnd equalizing their distributions (for details of the normalization, see LISS and QNORM entries in the Connectopedia glossary). Normalization: RNAseq RPKM values were log 2 transformed using log 2(max(RPKM, eps)). Feature Mapping: Ensembl Ids from the source data were mapped to Entrez Gene Ids using gene annotations from NCBI (downloaded on 0).
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